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Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

11.8K Views

13:15 min

February 25th, 2016

DOI :

10.3791/53417-v

February 25th, 2016


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Mitochondrial Localization

Rozdziały w tym wideo

0:05

Title

1:01

Sodium Carbonate Extraction of Intact Mitochondria

3:07

Separation of Inner and Outer Membranes of Mitochondria

4:22

Construction of Mitochondria-targeted GFP/RFP-tagged CyclinB1/Cdk1 Vectors and Mitochondria Localization

7:23

Determination of Cell Cycle Phase Lengths with EdU Incorporation Assay

10:58

Results: Mitochondrial Analysis of CyclinB1 and Cdk1

12:25

Conclusion

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