JoVE Logo

Zaloguj się

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

11.8K Views

13:15 min

February 25th, 2016

DOI :

10.3791/53417-v

February 25th, 2016


Przeglądaj więcej filmów

Mitochondrial Localization

Rozdziały w tym wideo

0:05

Title

1:01

Sodium Carbonate Extraction of Intact Mitochondria

3:07

Separation of Inner and Outer Membranes of Mitochondria

4:22

Construction of Mitochondria-targeted GFP/RFP-tagged CyclinB1/Cdk1 Vectors and Mitochondria Localization

7:23

Determination of Cell Cycle Phase Lengths with EdU Incorporation Assay

10:58

Results: Mitochondrial Analysis of CyclinB1 and Cdk1

12:25

Conclusion

Powiązane Filmy

article

08:29

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

13.8K Views

article

12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

18.5K Views

article

08:33

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

4.4K Views

article

10:47

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

2.9K Views

article

10:13

Methods to Assess Subcellular Compartments of Muscle in C. elegans

16.6K Views

article

09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

39.4K Views

article

08:33

Optimized Automated Analysis of Live Neuronal Mitochondria Homeostasis Modulation by Isoform-Specific Retinoic Acid Receptors

557 Views

article

09:53

Studying Mitochondrial Structure and Function in Drosophila Ovaries

23.9K Views

article

08:43

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

18.9K Views

article

08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

14.2K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone