Sodium Carbonate Extraction of Intact Mitochondria
3:07
Separation of Inner and Outer Membranes of Mitochondria
4:22
Construction of Mitochondria-targeted GFP/RFP-tagged CyclinB1/Cdk1 Vectors and Mitochondria Localization
7:23
Determination of Cell Cycle Phase Lengths with EdU Incorporation Assay
10:58
Results: Mitochondrial Analysis of CyclinB1 and Cdk1
12:25
Conclusion
Transcription
The overall goal of the following procedure is to determine the submitochondrial localization of a normally nuclear cell cycle kinase and then to analyze how its mitochondrial localization affects the progression of the cell cycle. This method can
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Here, we outline how to study mitochondrial localization of a (cell cycle) kinase, and how to determine its sub-mitochondrial location as well as potential mitochondrial substrates/targets. Forced expression of proteins into the mitochondria provides a useful tool for studying the functional consequences of mitochondrial localization of a protein of interest.