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High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

2.7K Views

10:47 min

May 5th, 2023

DOI :

10.3791/65236-v

May 5th, 2023


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High throughput

Rozdziały w tym wideo

0:00

Introduction

1:21

Preparation of siRNA Mixture for Mitochondrial DNA Synthesis

2:00

Cell Transfection and 5‐Bromo‐2’‐Deoxyuridine (BrdU) Incorporation in HeLa Cells

3:14

5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells

4:51

HeLa Cell Fixation with Hoechst 33342 and Blocking

5:51

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

7:49

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells

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