An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
Please note that all translations are automatically generated. Click here for the English version.
19.0K Views
•
08:40 min
•
March 14th, 2016
DOI :
March 14th, 2016
•Mohameedyaseen Syedbasha1, Janina Linnik1,2,3, Deanna Santer4, Daire O'Shea5, Khaled Barakat4,6, Michael Joyce4, Nina Khanna7, D. Lorne Tyrrell4, Michael Houghton4, Adrian Egli1,8
1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, and Swiss Institute of Bioinformatics, 3Swiss Institute of Bioinformatics, 4Li Ka Shing Institute for Virology, University of Alberta, 5Regional Infectious Diseases Unit, University of Edinburgh, 6Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 7Infection Biology, Department of Biomedicine, University of Basel, 8Clinical Microbiology, University Hospital Basel
Rozdziały w tym wideo
0:05
Title
1:07
Preparations and Introduction of Assays
2:14
Direct Ligand-Receptor-Interaction Assay (Direct LRA)
5:05
Competition Ligand-Receptor-Interaction Assay (Competition LRA)
6:24
Results: Binding Between INFL 1-3 Peptides and Their Receptor Sub-unit IL28RA
7:48
Conclusion
Powiązane Filmy
10:13
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
16.2K Views
12:38
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
19.7K Views
Optimized Methods for the Surface Immobilization of Collagens and Collagen Binding Assays
650 Views
10:16
Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
44.0K Views
06:56
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
13.6K Views
Real-Time Detection of Dynamic Affinity between Biomolecules Using Surface Plasmon Resonance (SPR) Technology
683 Views
02:13
Test wiązania in vitro oparty na SELEX w celu identyfikacji interakcji RNA-białko
207 Views
02:01
Metoda analizy oparta na teście ELISA do analizy interakcji między białkami przynęty i ofiary
743 Views
02:18
Stała wiązania równowagi i siła wiązania
12.8K Views
04:33
Konkurencyjny test wiązania w celu identyfikacji związków zakłócających interakcje receptor-ligand
84 Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone