Results: Binding Between INFL 1-3 Peptides and Their Receptor Sub-unit IL28RA
7:48
Conclusion
Transkript
The goals of this ELISA-based method are first to determine the binding affinity of a cytokine to its receptor and second, to measure the blocking effects of inhibitory peptides of the ligand-receptor interaction. This method can help answer key q
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The presented protocols describe two enzyme-linked immunosorbent assay (ELISA) based techniques for the rapid investigation of ligand-receptor interactions: The first assay allows the determination of dissociation constant between ligand and receptor. The second assay enables a rapid screening of blocking peptides for ligand-receptor interactions.