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A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

11.8K Views

11:44 min

January 24th, 2016

DOI :

10.3791/53723-v

January 24th, 2016


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Keywords Phosphorylation

Rozdziały w tym wideo

0:05

Title

1:01

Infection of A549 Cells

3:36

Preparation of Whole Cell Extracts

5:07

Resolution of Whole Cell Extracts by High Resolution SDS-PAGE

6:20

Analysis of IRF3 Dimerization by Native-PAGE

7:37

Immunoblot Analysis of IRF3 Species

9:23

Results: Detection Distinct Phosphorylated IRF3 Species

10:56

Conclusion

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