Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

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13.4K Views

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08:31 min

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October 18th, 2016

DOI :

10.3791/54296-v

October 18th, 2016

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Gloria Figueroa¹, Tiyash Parira¹, Alejandra Laverde¹, Gianna Casteleiro¹, Amal El-Mabhouh², Madhavan Nair¹, Marisela Agudelo¹

¹Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, ²Millipore Sigma

Podsumowanie

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Imaging Flow Cytometry

Cell Surface Expression

Rozdziały w tym wideo

0:05

Title

1:17

Standard Density Gradient Peripheral Blood Mononuclear Cells (PBMC) Isolation

2:24

Monocyte Purification

4:38

Cell Surface Staining Analysis by Imaging Flow Cytometry

5:46

Results: Representative Monocyte Yield and Isolation Analyses

7:22

Conclusion

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