Standard Density Gradient Peripheral Blood Mononuclear Cells (PBMC) Isolation
2:24
Monocyte Purification
4:38
Cell Surface Staining Analysis by Imaging Flow Cytometry
5:46
Results: Representative Monocyte Yield and Isolation Analyses
7:22
Conclusion
副本
The overall goal of this study is to compare two different methods of human monocyte isolation for dendritic cell generation in vitro and to characterize the CT11c, CT14 cell surface expression of the resulting monocyte derived populations by imag
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This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.