Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

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9.7K Views

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10:03 min

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November 11th, 2016

DOI :

10.3791/54375-v

November 11th, 2016

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Hidehito Saito¹^,², Kumiko Iwabuchi³, Noemi Fusaki⁴^,⁵, Fumito Ito³^,⁶

¹Department of Surgery, University of Michigan, ²Department of Biochemistry II, Kanazawa Medical University, ³Center for Immunotherapy, Roswell Park Cancer Institute, ⁴DNAVEC Corporation, ⁵Department of Ophthalmology, Keio University School of Medicine, ⁶Department of Surgical Oncology, Roswell Park Cancer Institute

Podsumowanie

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Keywords Induced Pluripotent Stem Cells

IPSCs

Melanoma

Tumor infiltrating Lymphocytes

Cancer Immunotherapy

T Cell Receptor Repertoire

Tumor Microenvironment

Tumor Dissociation

Density Gradient Separation

T cell Medium

IL 2

Rozdziały w tym wideo

0:05

Title

1:17

Tumor-infultrating Lymphocyte (TIL) Isolation and Culture

3:58

Mitomycin-C-treated SNL Feeder Cell Plate Preparation

5:28

Sendai Virus (SeV) Vector Induced Pluripotent Stem Cell (iPSC) Generation

7:55

Results: Representative TIL-derived iPSC Generation

9:18

Conclusion

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