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JoVE Journal

Developmental Biology

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Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

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10:03 min

November 11th, 2016

November 11th, 2016

9,670 Views

0:05

Title

1:17

Tumor-infultrating Lymphocyte (TIL) Isolation and Culture

3:58

Mitomycin-C-treated SNL Feeder Cell Plate Preparation

5:28

Sendai Virus (SeV) Vector Induced Pluripotent Stem Cell (iPSC) Generation

7:55

Results: Representative TIL-derived iPSC Generation

9:18

Conclusion

Transcription

The overall goal of this experiment is to generate induced pluripotent stem cells or iPSCs from melanoma infiltrating lymphocytes. This method can help answer key questions in the field of tumor immunology especially for cancer immunotherapy. The

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The goal of this protocol is to show the protocol for reprogramming melanoma tumor-infiltrating lymphocytes into induced pluripotent stem cells.

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