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The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

15.1K Views

08:26 min

December 14th, 2016

DOI :

10.3791/54582-v

December 14th, 2016


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ELISpot

Rozdziały w tym wideo

0:05

Title

0:48

Human Peripheral Blood B Cell Isolation and Purification

2:57

Naïve and Memory B Cell and Plasmablast/Plasma Cell Sorting

3:54

In Vitro Isolated Human CD19+, CD19+CD27+ Memory, and CD19+CD27- Naïve B Cell Differentiation and ELISpot Analysis

6:18

Results: Representative ELISpot Analysis

7:06

Conclusion

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