Human Peripheral Blood B Cell Isolation and Purification
2:57
Naïve and Memory B Cell and Plasmablast/Plasma Cell Sorting
3:54
In Vitro Isolated Human CD19+, CD19+CD27+ Memory, and CD19+CD27- Naïve B Cell Differentiation and ELISpot Analysis
6:18
Results: Representative ELISpot Analysis
7:06
Conclusion
Transcription
The overall goal of this ELISpot methodology is to allow the quantification of Human Antibody-secreting B cells from the blood. This method can help answer key questions in the bio-medical field about the immunology of B cells and vaccine research
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Human peripheral blood is commonly used for the assessment of the humoral immune response. Here, the methods for isolating human B cells from peripheral blood, differentiating human B cells into antibody (Ab)-secreting B cells (ASCs) in culture, and enumerating the total IgM- and IgG-ASCs via an ELISpot assay are described.