Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

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18.6K Views

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12:47 min

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December 27th, 2016

DOI :

10.3791/55001-v

December 27th, 2016

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Clément Danis*¹^,², Clément Despres*¹, Luiza M. Bessa¹, Idir Malki¹, Hamida Merzougui¹, Isabelle Huvent¹, Haoling Qi¹, Guy Lippens¹, François-Xavier Cantrelle¹, Robert Schneider¹, Xavier Hanoulle¹, Caroline Smet-Nocca¹, Isabelle Landrieu¹

¹UMR8576, CNRS, Lille University, ²UMR-S1172, INSERM CNRS, Lille University

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:15

Production of ¹⁵N, ¹³C-Tau

4:18

Purification of ¹⁵N, ¹³C-Tau

7:38

Acquisition of NMR Spectra

10:28

Results: Evaluation of the Purification Procedure of the Recombinant Protein and NMR Spectrum

11:35

Conclusion

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