Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

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21.2K Views

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13:16 min

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January 22nd, 2018

DOI :

10.3791/55451-v

January 22nd, 2018

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Seyyed Hamidreza Hashemipetroudi¹, Ghorbanali Nematzadeh¹, Gholamreza Ahmadian², Ahad Yamchi³, Markus Kuhlmann⁴

¹Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), ²Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), ³Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, ⁴RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

Podsumowanie

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Keywords DNA Contamination

RNA Samples

Ribosomal DNA

Poaceae

Quantitative Real time PCR

ITS Region

Aeluropus Littoralis

Genomic DNA

Denaturing Gel Electrophoresis

DNase I

RNase A

Rozdziały w tym wideo

0:05

Title

1:35

Nucleic Acid Extraction

4:27

Validation of rDNA Based Primers with DNA Templates

6:18

gDNA Contamination Assay with RNA Templates

7:49

RT-PCR Step for cDNA Synthesis and qPCR Analysis

9:55

Results: Use of rDNA Based Primers to Detect Genomic DNA in RNA Samples of LeadTissues

12:23

Conclusion

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