Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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27.4K Views

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13:34 min

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September 29th, 2012

DOI :

10.3791/3851-v

September 29th, 2012

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Garrett M. Dahm¹^,², Matthew M. Gubin¹^,², Joseph D. Magee², Patsharaporn Techasintana¹^,², Robert Calaluce², Ulus Atasoy¹^,²^,³

¹Molecular Microbiology and Immunology, University of Missouri, ²Department of Surgery, University of Missouri, ³Child Health, University of Missouri

Podsumowanie

Rozdziały w tym wideo

0:00

Title

1:43

Harvesting RNP Complex

3:59

Preparation of Isolation Materials

6:40

Immunoprecipitation

8:42

DNase and Proteinase K Treatment and RNA Precipitation

12:11

Representative Results

12:49

Conclusions

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