Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

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12:02 min

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June 6th, 2017

DOI :

10.3791/55745-v

June 6th, 2017

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Aintzane Apraiz*¹, Jone Mitxelena*²^,³, Ana Zubiaga²

¹Department of Cell Biology and Histology, University of the Basque Country, UPV/EHU, ²Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, ³Department of Molecular Mechanisms of Disease, University of Zurich

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Rozdziały w tym wideo

0:05

Title

11:07

Conclusion

9:02

Results: Thy-noc and HU Cell Synchronization Protocols Enable Analysis of Gene Expression During Specific Phases of the Cell Cycle

0:41

Thymidine- and Nocodazole-based (Thy-noc) Synchronization and Release

3:34

HU-based Synchronization and Release from G1/S Boundary

5:14

Collecting Samples for FACS Analysis and RNA Extraction

7:00

RNA Extraction for Gene Expression Analysis

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