Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

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06:32 min

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June 5th, 2017

DOI :

10.3791/55893-v

June 5th, 2017

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Naoki Ihara¹, Yuji Ikegaya¹^,², Haruki Takeuchi¹^,³

¹Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, ²Center for Information and Neural Networks, National Institute of Information and Communications Technology, ³PRESTO, Japan Science and Technology Agency (JST)

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Keywords Quadruple Immunostaining

Olfactory Bulb

Olfactory Sensory Axon

Molecular Identity Codes

Axon Sorting Molecules

Olfactory Development

Axon Guidance

Synapse Formation

Olfactory Sensory Neuron Regeneration

Olfactory Tissue Embedding

Rozdziały w tym wideo

0:05

Title

5:42

Conclusion

4:26

Results: Quantification of Axon-sorting Molecules in the Olfactory Bulb

1:11

Preparation of Parasagittal Olfactory Bulb Sections

2:33

Day 1: Quadruple Immunostaining of Olfactory Bulb Slices

3:07

Day 2: Quadruple Immunostaining of Olfactory Bulb Slices

3:56

The Use of Fluorescence Intensity Measurements

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