We would like to thank Scott Hamilton, and members of the O'Halloran lab for careful reading of this manuscript. We also thank our anonymous reviewer for excellent suggestions and insightful comments.

1. Construction of GFP Tagged EGL-4 Expressing Animals
<ol>
	<li>Clone the translational fusion GFP::EGL-4 under the promoter for the odr-3 gene (use 2678 bp directly upstream of the start codon): (p)odr-3::GFP::EGL-4. The odr-3 promoter drives expression in the amphid neuron pairs: AWA; AWB; AWC; and weakly in ASH.</li>
	<li>Inject the plasmid (p)odr-3::GFP::EGL-4 into wildtype (N2) animals at 50 ng/&mu;l with the co-injection markers using standard germ-line transformation techniques8: (p)ofm-1::GFP9 (25 ng/&mu;l), and (p)odr-1::dsRed10 (25 ng/&mu;l). The odr-1 promoter drives expression in the AWC and AWB amphid neuron pairs, and the ofm-1 promoter drives expression in the coelomocytes.</li>
	<li>Subject the transgenic animals expressing the extrachromosomal arrays detailed in Step 1.2 to the Ultra Violet (UV)/trimethylpsoralen (TMP) integration protocol11.</li>
	<li>Bleach synchronize the animals12 and cultivate them on Nematode Growth Media (NGM) plates containing OP50 E. coli to the L4 stage. Wash L4 animals off NGM plates with M9 buffer to remove OP50 E. coli bacteria.</li>
	<li>Remove M9 buffer and add 50-100 &mu;l of 30 &mu;g/ml TMP working solution onto the worm pellet in the 1.5 ml microcentrifuge tube. Wrap the 1.5 ml microcentrifuge tube in foil to block ambient light, and agitate gently on a rotator for 15 min in the foil-wrapped 1.5 ml microcentrifuge tube. Transfer the worms in the TMP/worm solution to a large unseeded NGM plate.</li>
	<li>Expose the worms on the NGM plate to UV 350 &mu;J(X100) using a Stratalinker 2400. Then add worms to an OP50 containing NGM plate and incubate in the dark for 5 hr.</li>
	<li>Pick 50 transgenic worms to approximately 10 seeded plates (2-5 worms each plate). Transfer P0's to new plates every 24 hr for 3 days.</li>
	<li>Pick 250 clonal F1 animals (1 animal per plate). Clone out 2-4 F2 animals from each F1 plate. Check F3 animals to look for a 100% transmission line by looking at (p)ofm-1::GFP expression pattern under a dissecting fluorescent microscope.</li>
	<li>Outcross the final integrated line(s) with wildtype N2 animals 5 times. We will refer to the final integrated line as pyIs500, and the GFP-tagged EGL-4 molecule as GFP::EGL-4.</li>
</ol>
2. Cultivation and Maintenance of Animals for Nuclear Translocation Assays
<ol>
	<li>Cultivate the pyIs500 animals at 25 &deg;C on standard 10 cm NGM plates (see below for recipe) seeded with OP50 E. coli bacteria13 (Figure 1).</li>
	<li>On Day 1, pick four to five L4 pyIs500 animals from plates cultivated at 25 &deg;C onto five separate 10 cm OP50 E. coli seeded plates and incubate at 25 &deg;C (that is, 4-5 L4 animals per plate).</li>
	<li>On Day 4, wash adult populations of pyIs500 animals off the large NGM plates using S-Basal buffer (see below for recipe). Use disposable glass pipettes to transfer animals, as they will stick to the side of plastic pipette tips.</li>
</ol>
3. Long-term Odor Induced Nuclear Translocation Assays
<ol>
	<li>Wash the pyIs500 animals 3 times in S-Basal to remove bacteria. Do not centrifuge animals between washes; rather allow the animals to settle by gravity in a stationary 1.5 ml microcentrifuge tube. It is critical to ensure all bacteria is removed, and that NGM pates are free from contamination, as residual bacteria will negatively affect the outcome of the translocation assay. Also, we have found that autoclaving the 1.5 ml microcentrifuge tubes produces a "sticky" property on the interior walls of the tube that causes animals to stick to the sides, and so use non-autoclaved 1.5 ml microcentrifuge tubes.</li>
	<li>While animals are settling between washes, make up the adaptation solution. Add 100 ml of S-Basal buffer to a 100 ml graduating cylinder. Add adapting odor to the S-Basal and seal the cylinder using a strip of Parafilm. If performing benzaldehyde adaptation add 7.5 &mu;l of benzaldehyde to 100 ml of S-Basal, for butanone adaptation add 11 &mu;l of 2-butaone to 100 ml of S-Basal. Gently invert (do not shake) the graduating cylinder containing S-Basal and odor 30 times to create a uniform emulsion.</li>
	<li>After the final wash in S-Basal, allow the animals to settle and remove all liquid. Label one tube "+ve" or "adapt" and the other tube "-ve" or "unadapt". Then add 1 ml of the adaptation mix to the adaptation microcentrifuge tube (+ve), and add 1 ml of S-Basal to the unadapted control microcentrifuge tube (-ve). Try to keep numbers of animals between 100 and 200 in each tube. With some practice, it is possible to estimate the approximate number of animals in a pellet by eye.</li>
	<li>Place a 1.5 ml microcentrifuge tube cap protector to each tube (prevents lids from popping open) and place the tubes on a rotator for 80 min. We have found that odor exposure between 60 min and 80 min yields similar results (both result in long-term adaptation responses). However, 80 min exposure provides more consistent results in our hands. Thus all our long-term adaptation assays are standardized by using an 80 min odor exposure (Figure 2).</li>
	<li>After 80 min, wash animals 3 times in S-Basal. Allow animals to settle by gravity between each wash.</li>
</ol>
4. Short-term Odor-induced Nuclear Translocation Assays
<ol>
	<li>In the case of short-term odor adaptation, use the same protocol as per long-term odor-induced translocation assays (Steps 3.1-3.4), however instead of incubating the pyIs500 animals in adapting odor for 80 min, use a 30 min exposure. The quantification of odor-induced nuclear translocation assays outlined below (Step 5) is the same for long-term and short-term exposures.</li>
</ol>
5. Scoring the Odor-induced Nuclear Translocation Event
<ol>
	<li>Make 2% agarose pads containing 5 mM sodium azide (NaN3) by dissolving gel electrophoresis agarose in dH20. Place a single drop of molten agarose containing NaN3 on a glass microscope slide. Immediately place another glass microscope slide on the first slide to flatten the drop of molten agarose. Leave for 30 sec, and then gently tease apart the microscope slides leaving one microscope slide with a flat agarose pad of ~1 mm.</li>
	<li>After the final wash in S-Basal, allow the worms to settle in the 1.5 ml microcentrifuge tube and remove all liquid. Then add the animals drop-wise onto the agarose pads of several microscope slides. It's important to keep the numbers of animals per slide to between 50 and 100. Too many animals per slide will complicate scoring and often create a scattering glow after blue-light excitation. Excess liquid may be wicked from the agarose pad by using a small Kim-wipe. Place a 0.5 mm glass cover-slip onto the pad and let stand for 2 min to allow for the NaN3 to immobilize the animals. Note, animals can be recovered from the slides after scoring. The animals will recover from the NaN3 anesthetic after ~1 hr if transferred to a seeded NGM plate into a drop of M9 Buffer.</li>
	<li>At this point it is imperative that each experimenter devise a key to track which slide is the experimental (+ve) slide and which slide is the control (-ve) slide, and pass the slides to another person that will blindly score the GFP:EGL-4 localization pattern. This will control for any experimenter bias.</li>
	<li>Score between 50 and 100 animals per slide using an upright fluorescent microscope with 10X, 40X and 63X magnification objectives and GFP/RFP filters. Firstly, locate animals under 10X magnification using bright-field illumination. Secondly, locate the AWC neuron by turning off the bright-field illumination and using the RFP filter. The (p)odr-1::dsRed drives expression in AWB and AWC neurons. The AWC neurons have distinctive oval shaped cell bodies as compared with AWB cell bodies which are smaller and circular in shape (Figure 2).</li>
	<li>Once the AWC cell body has been located, switch to the GFP filter and record the localization of GFP:EGL-4 as nuclear or cytoplasmic. Using a counter allows the experimenter to keep looking into the eyepiece while scoring the slide. Note: Steps 5.1-5.5 are repeated at least 3 times on separate days to produce statistically significant data (Figures 1-2).</li>
</ol>
6. Monitoring GFP-tagged EGL-4 during the Recovery from Long-term Odor Adaptation
<ol>
	<li>After Step 3.5, divide the populations of pyIs500 animals into multiple aliquots and score for nuclear versus cytoplasmic GFP::EGL-4 in the AWC at time point zero (that is, after 80 min exposure) and multiple subsequent time points (Figure 3).</li>
	<li>Transfer both the control (-ve) and experimental (+ve) animals using disposable glass pipettes onto NGM plates.</li>
	<li>Allow animals to recover for various lengths of time and re-examine the localization of GFP::EGL-4in AWC at each time point as in Steps 5.1 to 5.5.</li>
</ol>
7. Materials
NGM plates: For 1 L add to 1 L ddH20: 21 g Bacto-Agar; 3 g Sodium Chloride (NaCl); 2.5 g Bacto-Peptone. Autoclave Media. After autoclaving, cool the agar until the temperature reads 54 &deg;C. Add the following solutions: 25 ml 1M Potassium Phosphate Buffer; 1 ml 1 M Magnesium Sulfate (MgSO4); 1 ml 1 M Calcium Chloride (CaCl2); 1 ml Cholesterol in Ethanol (5 mg/ml stock).
1 M Dibasic K2HPO4 174.2 g/mol: For 500 ml: In 400 ml ddH2O, dissolve 87.1 g Dibasic K2HPO4. Adjust volume to 500 L with ddH2O. Filter sterilize using a 500 ml Sterile Filter Unit.
1 M Monobasic KH2PO4 136.1 g/mol: For 1 L: In 800 ml ddH2O, dissolve 136.1 g Monobasic KH2PO4. Adjust volume to 1 L with ddH2O. Filter sterilize using a 500 ml Sterile Filter Unit.
1 M Potassium Phosphate (K3PO4) Buffer pH 6.0: For 1 L: In a 1 L Sterile Filter Unit, add 868 ml of 1 M Monobasic KH2PO4. Allow unit to filter through, then add 132 ml of 1 M Dibasic K2HPO4. Allow unit to filter all the liquid. Remove filter unit and adjust pH to 6.0. Store at room temperature.
S-Basal: For 1 L: add 50 ml 1M K3PO4 Buffer, pH ~6.0; and 20 ml 5M NaClaq. Fill to 1 L with ddH2O. Autoclave Solution.
M9: For 1 L: 3 g KH2PO4, 6 g Na2HPO4, 5 g NaCl, 1 ml 1 M MgSO4, H2O. Fill to 1 L with ddH20. Sterilize by autoclaving.

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	<li>Colbert, H. A., Bargmann, C. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Odorant-specific+adaptation+pathways+generate+olfactory+plasticity+in+C.+elegans.">Odorant-specific adaptation pathways generate olfactory plasticity in C. elegans.</a> Neuron. 14 (4), 803 (1995).</li><li>Colbert, H. A., Bargmann, C. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Environmental+signals+modulate+olfactory+acuity,+discrimination,+and+memory+in+Caenorhabditis+elegans.">Environmental signals modulate olfactory acuity, discrimination, and memory in Caenorhabditis elegans.</a> Learning and Memory. 4 (2), 179 (1997).</li><li>Bargmann, C. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Odorant-selective+genes+and+neurons+mediate+olfaction+in+C.+elegans.">Odorant-selective genes and neurons mediate olfaction in C. elegans.</a> Neuron. 74 (3), 515 (1993).</li><li>L'Etoile, N. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+cyclic+GMP-dependent+protein+kinase+EGL-4+regulates+olfactory+adaptation+in+C.+elegans.">The cyclic GMP-dependent protein kinase EGL-4 regulates olfactory adaptation in C. elegans.</a> Neuron. 36, 1079-10 (2002).</li><li>Lee, J. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nuclear+entry+of+a+cGMP-dependent+kinase+converts+transient+into+long-lasting+olfactory+adaptation.">Nuclear entry of a cGMP-dependent kinase converts transient into long-lasting olfactory adaptation.</a> PNAS. 107 (13), 6016 (2010).</li><li>O'Halloran, D. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulators+of+AWC-mediated+olfactory+plasticity+in+Caenorhabditis+elegans.">Regulators of AWC-mediated olfactory plasticity in Caenorhabditis elegans.</a> PLoS Genetics. 5 (12), e1000761 (2009).</li><li>O'Halloran, D. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+cGMP+levels+affect+the+localization+of+EGL-4+in+AWC+in+Caenorhabditis+elegans.">Changes in cGMP levels affect the localization of EGL-4 in AWC in Caenorhabditis elegans.</a> PLoS ONE. 7 (2), e31614 (2012).</li><li>Mello, C. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficient+gene+transfer+in+C.elegans:+extrachromosomal+maintenance+and+integration+of+transforming+sequences.">Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.</a> EMBO Journal. 10, 3959-39 (1991).</li><li>Miyabayashi, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+and+function+of+members+of+a+divergent+nuclear+receptor+family+in+Caenorhabditis+elegans.">Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans.</a> Dev. Biol. 215, 314 (1999).</li><li>L'Etoile, N. D., Bargmann, C. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Olfaction+and+odor+discrimination+are+mediated+by+the+C.+elegans+guanylyl+cyclase+ODR-1.">Olfaction and odor discrimination are mediated by the C. elegans guanylyl cyclase ODR-1.</a> Neuron. 25 (3), 575 (2000).</li><li>Mello, C., Fire, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=DNA+transformation.">DNA transformation.</a> Methods Cell Biol. 48, 451 (1995).</li><li>Stiernagle, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Maintenance+of+C.+elegans.">Maintenance of C. elegans.</a> Wormbook. , (2006).</li><li>Brenner, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+genetics+of+Caenorhabditis+elegans.">The genetics of Caenorhabditis elegans.</a> Genetics. 77 (1), 71 (1974).</li><li>Kauffman, A., Parsons, L., Stein, G., Wills, A., Kaletsky, R., Murphy, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=C.+elegans+Positive+Butanone+Learning,+Short-term,+and+Long-term+Associative+Memory+Assays.">C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays.</a> J. 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No conflicts of interest declared.

The odor-induced nuclear entry of a GFP-tagged EGL-4 molecule described here provides a robust molecular readout of olfactory adaptation in C. elegans. The odor-induced nuclear translocation assays are straightforward and require only a few days of preparation time. The pyIs500 animal that we have constructed for these assays, expresses a marker that illuminates the AWC neuron as well as expressing the GFP-tagged EGL-4 protein. Thus, we feel that an experimenter with little to no experience working with...

<table border="1">
	<tbody>
		<tr>
			<td>Name of the reagent</td>
			<td>Company</td>
			<td>Catalogue number</td>
			<td>Comments </td>
		</tr>
		<tr>
			<td>Bacto Agar</td>
			<td>Difco</td>
			<td>DF0140-07-4</td>
			<td>NGM plates</td>
		</tr>
		<tr>
			<td>Sodium Chloride</td>
			<td>Fisher Chemical</td>
			<td>S671-10</td>
			<td>NGM plates</td>
		</tr>
		<tr>
			<td>Bacto Peptone</td>
			<td>Difco</td>
			<td>DF0118-07-2</td>
			<td>NGM plates</td>
		</tr>
		<tr>
			<td>Potassium Phosphate Dibasic</td>
			<td>Fisher Chemical</td>
			<td>S375-500</td>
			<td>S-Basal buffer and NGM plates</td>
		</tr>
		<tr>
			<td>Potassium Phosphate Monobasic</td>
			<td>Fisher Chemical</td>
			<td>P285-500</td>
			<td>S-Basal buffer and NGM plates</td>
		</tr>
		<tr>
			<td>Kimwipes - Small</td>
			<td>Kimberly-Clark</td>
			<td>LS2770</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Ethanol 100%</td>
			<td>Gold Shield Chemical Co.</td>
			<td>43196-115</td>
			<td>diluting odors for chemotaxis assays</td>
		</tr>
		<tr>
			<td>Calcium Chloride</td>
			<td>Sigma-Aldrich</td>
			<td>C8106-500G</td>
			<td>NGM plates</td>
		</tr>
		<tr>
			<td>Magnesium Sulphate</td>
			<td>MP Biomedicals</td>
			<td>150136-500G</td>
			<td>NGM plates</td>
		</tr>
		<tr>
			<td>Sodium Azide 99%</td>
			<td>Fisher Scientific</td>
			<td>ICN10289180</td>
			<td>Anesthetic</td>
		</tr>
		<tr>
			<td>Agarose - UltraPure</td>
			<td>Invitrogen</td>
			<td>16500-500</td>
			<td>Agarose pads</td>
		</tr>
		<tr>
			<td>Benzaldehyde</td>
			<td>Sigma-Aldrich</td>
			<td>B1334-100G</td>
			<td>AWC odor</td>
		</tr>
		<tr>
			<td>Butanone, ACS Grade</td>
			<td>Sigma-Aldrich</td>
			<td>360473-500ML</td>
			<td>AWC odor</td>
		</tr>
		<tr>
			<td>Microcentrifuge Tubes - 1.5 ml Colored</td>
			<td>Denville</td>
			<td>LS8147</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Pasteur Pipet Disposable Glass 5-3/4"</td>
			<td>Fisher Scientific</td>
			<td>13-678-20B</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Stratalinker</td>
			<td>Stratagene</td>
			<td>Stratalinker 2400</td>
			<td>UV integration</td>
		</tr>
		<tr>
			<td>Filter Vacuum Bottle - 500 ml</td>
			<td>Nalgene</td>
			<td>09-740-25B</td>
			<td>&nbsp;</td>
		</tr>
	</tbody>
</table>

a molecular readout of long-term olfactory adaptation in c. elegans

During sustained stimulation most sensory neurons will adapt their response by decreasing their sensitivity to the signal. The adaptation response helps shape attention and also protects cells from over-stimulation. Adaptation within the olfactory circuit of C. elegans was first described by Colbert and Bargmann1,2. Here, the authors defined parameters of the olfactory adaptation paradigm, which they used to design a genetic screen to isolate mutants defective in their ability to adapt to volatile odors sensed by the Amphid Wing cells type C (AWC) sensory neurons. When wildtype C. elegans animals are exposed to an attractive AWC-sensed odor3 for 30 min they will adapt their responsiveness to the odor and will then ignore the adapting odor in a chemotaxis behavioral assay for ~1 hr. When wildtype C. elegans animals are exposed to an attractive AWC-sensed odor for ~1 hr they will then ignore the adapting odor in a chemotaxis behavioral assay for ~3 hr. These two phases of olfactory adaptation in C. elegans were described as short-term olfactory adaptation (induced after 30 min odor exposure), and long-term olfactory adaptation (induced after 60 min odor exposure). Later work from L'Etoile et al.,4 uncovered a Protein Kinase G (PKG) called EGL-4 that is required for both the short-term and long-term olfactory adaptation in AWC neurons. The EGL-4 protein contains a nuclear localization sequence that is necessary for long-term olfactory adaptation responses but dispensable for short-term olfactory adaptation responses in the AWC4. By tagging EGL-4 with a green fluorescent protein, it was possible to visualize the localization of EGL-4 in the AWC during prolonged odor exposure. Using this fully functional GFP-tagged EGL-4 (GFP::EGL-4) molecule we have been able to develop a molecular readout of long-term olfactory adaptation in the AWC5. Using this molecular readout of olfactory adaptation we have been able to perform both forward and reverse genetic screens to identify mutant animals that exhibit defective subcellular localization patterns of GFP::EGL-4 in the AWC6,7. Here we describe: 1) the construction of GFP::EGL-4 expressing animals; 2) the protocol for cultivation of animals for long-term odor-induced nuclear translocation assays; and 3) the scoring of the long-term odor-induced nuclear translocation event and recovery (re-sensitization) from the nuclear GFP::EGL-4 state.

An example of the localization pattern of GFP::EGL-4in the AWC before and after prolonged odor exposure is shown in Figure 2. Prior to prolonged odor exposure, GFP::EGL-4 is localized to the cytosol of the AWC (Figure 2B), and after 80 min odor exposure GFP::EGL-4 is localized to the nucleus of the AWC (Figure 2D). At the behavioral level, animals with cytosolic GFP::EGL-4 in AWC are attracted to a point source of odor (Figure 2C graphs representative re...

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A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

Here we describe a molecular readout of long-term olfactory adaptation in Caenorhabditis elegans. The Protein Kinase G, EGL-4, is necessary for stable adaptation responses in the primary sensory neuron pair called AWC. During prolonged odor exposure EGL-4 translocates from the cytosol to nucleus of the AWC.

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

During sustained stimulation most sensory neurons will adapt their response by decreasing their sensitivity to the signal. The adaptation response helps ...

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10.7K Views.  George Washington University. Here we describe a molecular readout of long-term olfactory adaptation in Caenorhabditis elegans. The Protein Kinase G, EGL-4, is necessary for stable adaptation responses in the primary sensory neuron pair called AWC. During prolonged odor exposure EGL-4 translocates from the cytosol to nucleus of the AWC.