Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
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12.8K Views
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11:29 min
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July 10th, 2017
DOI :
July 10th, 2017
•Frank J. Jenkins1,2, Charles M. Kerr2, Elise Fouquerel2,3, Dana H. Bovbjerg2,4, Patricia L. Opresko2,3
1Departments of Pathology and Infectious Diseases and Microbiology, University of Pittsburgh, 2University of Pittsburgh Cancer Institute, 3Department of Environmental and Occupational Health, University of Pittsburgh, 4Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences, University of Pittsburgh
Rozdziały w tym wideo
0:05
Title
0:46
Genomic DNA Digestion
1:39
Genomic DNA Gel Electrophoresis
2:56
Gel Fluorescent Imaging and Hybridization
5:17
Hybridization with Telomere Probe
7:36
Gel Washing, Phospher Screen Exposure, and Radio Imaging
9:08
Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis
10:28
Conclusion
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