Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

12.8K Views

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11:29 min

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July 10th, 2017

DOI :

10.3791/56001-v

July 10th, 2017

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Frank J. Jenkins¹^,², Charles M. Kerr², Elise Fouquerel²^,³, Dana H. Bovbjerg²^,⁴, Patricia L. Opresko²^,³

¹Departments of Pathology and Infectious Diseases and Microbiology, University of Pittsburgh, ²University of Pittsburgh Cancer Institute, ³Department of Environmental and Occupational Health, University of Pittsburgh, ⁴Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences, University of Pittsburgh

Summary

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Keywords Telomere Length

Modified Terminal Restriction Fragment Analysis

Agarose Gel Electrophoresis

Chapters in this video

0:05

Title

0:46

Genomic DNA Digestion

1:39

Genomic DNA Gel Electrophoresis

2:56

Gel Fluorescent Imaging and Hybridization

5:17

Hybridization with Telomere Probe

7:36

Gel Washing, Phospher Screen Exposure, and Radio Imaging

9:08

Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis

10:28

Conclusion

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