Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
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12:30 min
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October 6th, 2017
DOI :
October 6th, 2017
•Rozdziały w tym wideo
0:05
Title
1:27
Designing an Artificial Intronic Small RNA Expression Cassette
4:22
Generating a rAaeDV Construct by Cloning a miRNA or shRNA Expression Cassette into a Plasmid Backbone
5:19
Transfecting C6/36 Cells with rAaeDV Plasmids
7:33
Harvesting rAaeDV Virions from Transfected C6/36 Cells
8:53
Mosquito Transduction
10:10
Results: Overexpression and Knockdown Efficiency of rAaeDV Vectors
11:14
Conclusion
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