A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

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11.0K Views

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10:32 min

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December 6th, 2017

DOI :

10.3791/56264-v

December 6th, 2017

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Anna Köferle¹, Stefan H. Stricker¹^,²

¹MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian-Universität, BioMedical Center, ²Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health

Podsumowanie

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DNA Fragment Extraction

CORALINA Method

DNA Source Agnostic

Rozdziały w tym wideo

0:05

Title

0:48

Digestion of DNA with MNase and Separation of DNA Fragments by PAGE

3:05

Isolation of DNA Fragments from PAGE Gels and Linker Generation

4:26

End Repair of Gel-purified Fragments and Linker Ligation

5:32

Size Selection and Assembly of Vector-insert Construct

6:18

Electroporation of TG1 Electrocompetent E. coli Cells

8:38

Results: a gRNA Library can be Generated from and Target DNA

9:56

Conclusion

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