A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
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10:32 min
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December 6th, 2017
DOI :
December 6th, 2017
•Rozdziały w tym wideo
0:05
Title
0:48
Digestion of DNA with MNase and Separation of DNA Fragments by PAGE
3:05
Isolation of DNA Fragments from PAGE Gels and Linker Generation
4:26
End Repair of Gel-purified Fragments and Linker Ligation
5:32
Size Selection and Assembly of Vector-insert Construct
6:18
Electroporation of TG1 Electrocompetent E. coli Cells
8:38
Results: a gRNA Library can be Generated from and Target DNA
9:56
Conclusion
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