An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
Please note that all translations are automatically generated. Click here for the English version.
6.6K Views
•
11:53 min
•
June 1st, 2018
DOI :
June 1st, 2018
•Rozdziały w tym wideo
0:04
Title
0:57
Grow Mouse ES Cells on Gelatin-coated Dishes
2:48
Extract DNA from Cells and Enzymatic Digestion of DNA into Single Nucleosides
5:29
Analyze 5mC and 5hmC Using UHPLC-MS/MS
8:34
Results: Methylation Status of Mouse Embryonic Stem Cells
10:17
Conclusion
Powiązane Filmy
09:32
Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
10.2K Views
08:27
A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
6.3K Views
15:13
Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
10.9K Views
09:04
Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
9.0K Views
12:59
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
8.4K Views
09:38
Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
90.8K Views
11:56
Generation of Mice Derived from Induced Pluripotent Stem Cells
21.5K Views
12:26
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
13.8K Views
08:27
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
12.3K Views
08:01
A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
2.2K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone