Extract DNA from Cells and Enzymatic Digestion of DNA into Single Nucleosides
5:29
Analyze 5mC and 5hmC Using UHPLC-MS/MS
8:34
Results: Methylation Status of Mouse Embryonic Stem Cells
10:17
Conclusion
副本
The overall goal of this protocol is to use the small molecule compounds PD0325901 and Vitamin C to maintain a hypomethylated and pluripotent state in mouse embryonic stem cells. This method can help answer key questions in the field of FBS cultur
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We described in detail two chemical-based protocols for culturing mouse embryonic stem cells. This new method utilizes synergistic mechanisms of promoting Tet-mediated oxidation (by vitamin C) and repressing de novo synthesis of 5-methylcytosine (by PD0325901) to maintain DNA hypomethylation and pluripotency of mouse embryonic stem cells.