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Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

7.9K Views

10:28 min

November 30th, 2017

DOI :

10.3791/56836-v

November 30th, 2017


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In Vitro Assay

Rozdziały w tym wideo

0:05

Title

1:05

Bleach and Dechorionate 48 hpf Zebrafish Embryos

3:19

Embryos for Dissociation

5:15

Preparation of Dissociated Embryos

6:21

Preparation of Methylcellulose

7:30

Addition of Dissociated Embryos to Methylcellulose

8:53

Results: Quantitation of Zebrafish Embryonic Hematopoietic Stem and Progenitor Cells

9:32

Conclusion

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