Addition of Dissociated Embryos to Methylcellulose
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Results: Quantitation of Zebrafish Embryonic Hematopoietic Stem and Progenitor Cells
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Conclusion
文字起こし
The overall goal of this assay is to enumerate and examine the differentiation of hematopoietic progenitor cells in the developing zebrafish embryo. This method can help answer some really important questions in the hematopoietic field, such as, h
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Here, we present a simple method to quantitate hematopoietic stem and progenitor cells (HSPCs) in embryonic zebrafish. HSPCs from dissociated zebrafish are plated in methylcellulose with supportive factors, differentiating into mature blood. This allows the detection of blood defects and allows drug screening to be easily conducted.