Zaloguj się

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

8.5K Views

10:59 min

May 21st, 2018

DOI :

10.3791/56842-v

May 21st, 2018


Przeglądaj więcej filmów

Bacterial Lipoproteins

Rozdziały w tym wideo

0:04

Title

0:47

Cell Growth and Lysis

2:23

Enrichment of Lipoproteins by TX-114 Phase Partitioning

5:05

SDS-PAGE, Electroblotting, and Staining with Ponceau S

6:30

Tryptic Digestion, Lipopeptide Extraction from Nitrocellulose Membrane, Deposition onto MALDI Target, and Data Acquisition

8:47

Results: MALDI-TOF MS Analysis of N-terminal Lipopeptides

10:17

Conclusion

Powiązane Filmy

article

12:57

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

31.6K Views

article

08:59

Defining Substrate Specificities for Lipase and Phospholipase Candidates

14.9K Views

article

11:18

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography

16.1K Views

article

10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

10.0K Views

article

11:59

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

9.6K Views

article

08:46

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

3.7K Views

article

04:22

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

3.9K Views

article

High-Resolution Studies of Proteins in Natural Membranes by Solid-State NMR

2.2K Views

article

11:46

Shotgun Lipidomics of Rodent Tissues

1.9K Views

article

00:11

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

23.5K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone