Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
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8.6K Views
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10:59 min
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May 21st, 2018
DOI :
May 21st, 2018
•Rozdziały w tym wideo
0:04
Title
0:47
Cell Growth and Lysis
2:23
Enrichment of Lipoproteins by TX-114 Phase Partitioning
5:05
SDS-PAGE, Electroblotting, and Staining with Ponceau S
6:30
Tryptic Digestion, Lipopeptide Extraction from Nitrocellulose Membrane, Deposition onto MALDI Target, and Data Acquisition
8:47
Results: MALDI-TOF MS Analysis of N-terminal Lipopeptides
10:17
Conclusion
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