Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

The overall goal of this procedure is to provide readers detailed hands-on experience in constructing a phage displayed synthetic human antibody library with tailored diversity. Synthetic phage displayed antibody libraries have great potential for replace traditionally hybridoma-based techniques for therapeutic monoclonal antibody development by bypassing the need for animal immunization and the subsequent antibody humanization. The advantage of synthetic antibody library is that tailored diversity is introduced into an antibody framework using insight into positional diversity that support high affinity interaction and desirable protein properties.

Beyond the practical utility, synthetic antibody libraries can also be used to experimentally probe the role of specific amino acids in biomolecular interactions, thus supporting their own continuous improvement. This procedure will be demonstrated by graduate student named Zhenwei Zhong and Doctor Shin-chen Hou from our laboratory. To be