Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

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15.2K Views

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10:54 min

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April 4th, 2018

DOI :

10.3791/57400-v

April 4th, 2018

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Elisabeth Naschberger¹, Daniela Regensburger¹, Clara Tenkerian¹, Melanie Langheinrich², Felix B. Engel³, Carol Geppert⁴, Arndt Hartmann⁴, Robert Grützmann², Vera S. Schellerer*², Michael Stürzl*¹

¹Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, ²Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, ³Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, ⁴Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg

Podsumowanie

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Keywords Endothelial Cells

Tissue Microdissection

Cell Isolation Protocol

Rozdziały w tym wideo

0:04

Title

0:51

Surgery, Preparation of Tissue, and Generation of Single Cell Suspension

4:17

FACS Sorting, Harvesting and Characterization of Endothelial Cells

7:17

Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers

8:59

Conclusion

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