Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

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1.9K Views

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05:26 min

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February 3rd, 2023

DOI :

10.3791/64885-v

February 3rd, 2023

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Roberto Plebani¹^,², Alessandra D'Alessandro², Paola Lanuti²^,³, Pasquale Simeone²^,³, Massimiliano Cinalli¹, Ilaria Righi⁴, Alessandro Palleschi⁴^,⁵, Matteo Mucci¹^,², Marco Marchisio²^,³, Francesco Cappabianca¹, Marina Camera⁶^,⁷, Felice Mucilli¹, Mario Romano¹^,²

¹Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, ²Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, ³Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, ⁴U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, ⁵Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, ⁶Department of Pharmaceutical Sciences, Università degli Studi di Milano, ⁷Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS

Podsumowanie

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Microvascular Endothelial Cells

Macrovascular Endothelial Cells

Cell Isolation

Purification Protocol

Lung derived Samples

PBS phosphate buffered Saline

Collagenase Incubation

Rozdziały w tym wideo

0:04

Introduction

0:45

Sample Preparation and Enzymatic Digestion

1:47

Recovery of the Digested Cells and Removal of Non-Endothelial Cells (EC) by Filtration

2:32

Sedimentation of Cell Clusters and Cell Seeding

3:16

Results: Purification and Characterization of Freshly Isolated HLMVECs

4:30

Conclusion

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