Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
15.2K Views
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10:54 min
•
April 4th, 2018
DOI :
April 4th, 2018
•Elisabeth Naschberger1, Daniela Regensburger1, Clara Tenkerian1, Melanie Langheinrich2, Felix B. Engel3, Carol Geppert4, Arndt Hartmann4, Robert Grützmann2, Vera S. Schellerer*2, Michael Stürzl*1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg
Chapters in this video
0:04
Title
0:51
Surgery, Preparation of Tissue, and Generation of Single Cell Suspension
4:17
FACS Sorting, Harvesting and Characterization of Endothelial Cells
7:17
Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers
8:59
Conclusion
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