A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
Please note that all translations are automatically generated. Click here for the English version.
7.5K Views
•
08:37 min
•
April 30th, 2018
DOI :
April 30th, 2018
•Rozdziały w tym wideo
0:04
Title
0:42
sgRNA Target Selection and Homology-directed Repair Template Design
2:21
Prepare Injection Mix and Injection Protocol
3:18
Screen P0 Plates and Single mCherry(+) F1s
4:03
Single Worm PCR and Genotyping
5:41
Indentification and Sequence Verification of Edited Animals
6:34
Results: Rapid CRISPR/Cas9 Generated Point Mutations in C. elegans sod-1
7:50
Conclusion
Powiązane Filmy
14:46
Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
11.0K Views
08:25
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
13.7K Views
11:27
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
21.8K Views
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
33.6K Views
10:00
A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
9.7K Views
09:04
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
8.1K Views
09:16
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
7.3K Views
10:30
The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
9.5K Views
07:46
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
5.7K Views
02:44
PCR z pojedynczym robakiem: metoda ekstrakcji i amplifikacji genomowego DNA
4.6K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone