Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

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9.2K Views

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13:47 min

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June 3rd, 2018

DOI :

10.3791/57576-v

June 3rd, 2018

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Caleb Sutherland*¹, Yu Wang*², Robert V. Brown*¹, Julie Foley², Beth Mahler², Kyathanahalli S. Janardhan²^,³, Ramesh C. Kovi²^,⁴, Anton M. Jetten¹

¹Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH, ²Cellular and Molecular Pathology Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, NIH, ³Integrated Laboratory Systems Inc., ⁴Experimental Pathology Laboratories Inc.

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Keywords Laser Microdissection

Trabecular Meshwork

Gene Expression Analysis

Laser Capture Microscopy

Rozdziały w tym wideo

0:05

Title

1:00

Frozen Section Preparation for Laser Microdissection

4:25

Polyethylene Terephthalate (PET) Membrane Slides Processing and Staining Protocol

6:14

Laser Microdissection with UV Laser

11:01

Results: Evaluation of RNA Quality and Downstream RNA Analysis

12:25

Conclusion

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