Frozen Section Preparation for Laser Microdissection
4:25
Polyethylene Terephthalate (PET) Membrane Slides Processing and Staining Protocol
6:14
Laser Microdissection with UV Laser
11:01
Results: Evaluation of RNA Quality and Downstream RNA Analysis
12:25
Conclusion
Transkript
The overall goal of this laser microdissection procedure is to develop a robust and reproducible method for the isolation of a highly-enriched trabecular meshwork from mouse eyes using laser capture microscopy for subsequent RNA isolation and down
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Here, we describe a protocol for a reproducible laser capture microdissection (LCM) for isolating trabecular meshwork (TM) for downstream RNA analysis. The ability to analyze changes in gene expression in the TM will help in understanding the underlying molecular mechanisms of TM-related ocular diseases.