Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

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09:00 min

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April 16th, 2018

DOI :

10.3791/57584-v

April 16th, 2018

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Yoshihide Tokunou¹, Kazuhito Hashimoto², Akihiro Okamoto²

¹Department of Applied Chemistry, University of Tokyo, ²Global Research Center for Environment and Energy based on Nanomaterials Science, National Institute for Materials Science

Podsumowanie

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Keywords Deuterium Kinetic Isotope Effect

Extracellular Electron Transport

Shewanella Oneidensis MR 1

Electrochemical Detection

Proton Transport

C type Cytochromes

Three electrode Electrochemical Reactor

Rozdziały w tym wideo

0:05

Title

1:00

Formation of a Monolayer Biofilm of S. oneidensis MR-1 on an ITO Electrode

4:11

Replacement of the Supernatant with Fresh DM Medium with 10 mM Lactate

5:24

Addition of Deuterium Water to Measure the KIE on the EET Process

6:26

Results: Microbial Current Change Induced by D₂O Addition and Confirmation of Rate-limitation by Extracellular Electron Transport Process

7:36

Conclusion

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