<h2 class="jove_title"><a href="https://www.jove.com/video/53929">Modified Drop Tower Impact Tests for American Football Helmets</a></h2>G. Alston Rush1,2, R. Prabhu1, Gus A. Rush III3, Lakiesha N. Williams1, M. F. Horstemeyer2,41Agricultural and Biological Engineering, Mississippi State University, 2Center for Advanced Vehicular Systems, Mississippi State University, 3Rush Sport Medical, 4Mechanical Engineering, Mississippi State UniversityThis article provides a novel technique to assess the performance characteristics of American football helmets by inclusion of faceguards during NOCSAE Standard drop tests. Additionally, two more impact locations are proposed to be added to the NOCSAE certification. <h2 class="jove_title"><a href="https://www.jove.com/video/54097">Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates</a></h2>Geetika Sahni*1, Jun Yuan*1, Yi-Chin Toh1,21Department of Biomedical Engineering, National University of Singapore, 2Singapore Institute of Neurotechnology (SINAPSE), National University of SingaporeHuman pluripotent stem cells (hPSCs) have the intrinsic ability to differentiate and self-organize into distinct tissue patterns; although this requires the presentation of spatial environmental gradients. We present stencil micropatterning as a simple and robust method to generate biochemical and mechanical gradients for controlling hPSC differentiation patterns.<h2 class="jove_title"><a href="https://www.jove.com/video/54118">A Bending Test for Determining the Atterberg Plastic Limit in Soils</a></h2>Jos&#233; Manuel Moreno-Maroto, Jacinto Alonso-Azc&#225;rateDepartment of Physical Chemistry, Faculty of Environmental Sciences and Biochemistry, University of Castilla-La ManchaThe traditional standardized test for determining the plastic limit in soils is performed by hand, and the result varies depending on the operator. An alternative method based on bending measurements is presented in this study. This allows the plastic limit to be obtained with a clear and objective criterion.<h2 class="jove_title"><a href="https://www.jove.com/video/53981">Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements</a></h2>Wenming Bai1, Norio Yoshimura1, Masao Takayanagi1, Jingai Che2, Naomi Horiuchi2, Isao Ogiwara31United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 2Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3Institute of Agriculture, Tokyo University of Agriculture and TechnologyWe present here a protocol to construct and validate models for nondestructive prediction of total sugar, total organic acid, and total anthocyanin content in individual blueberries by near-infrared spectroscopy.

june 2016 - this month in jove: blueberry analysis, impact testing football helmets, stencil micropatterning of stem cells, and a test for soil plasticity

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/112">June 2016 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
In <a href="http://www.jove.com/chem">JoVE Chemistry</a>, just in time for blueberry season, we feature a method for predicting the constituents of blueberries while keeping them intact. <a href="http://www.jove.com/video/53981">Bai et al.</a> use near-infrared spectroscopy to obtain spectra of individual blueberries, then use high-performance liquid chromatography (HPLC) to measure their actual contents. They build models that use the near-infrared spectra to predict the levels of sugar, the organic acids that impart tartness, and the anthocyanins that have antioxidant properties. This method aids the selection and distribution of only the most delicious blueberries with guaranteed qualities.
<a href="http://www.jove.com/eng">JoVE Engineering</a> includes a test protocol that simulates on-field impacts of football helmets to help reduce the risks of head injuries. Current performance standards for football helmet certification don't require the faceguard to be attached during impact tests; however, attaching a faceguard dramatically changes the helmet's mechanical properties. Therefore, <a href="http://www.jove.com/video/53929/">Rush et al.</a> propose a modified test that includes the faceguard. They also test two new helmet impact locations. This method provides more robust helmet safety standards that will drive safer helmet designs.
In <a href="http://www.jove.com/devbio">JoVE Developmental Biology</a>, human pluripotent stem cells (hPSCs) can differentiate into all three developmental germ layers. However, in vitro differentiation of hPSCs tends to be a disorganized process. This month, <a href="http://www.jove.com/video/54097/">Sahni et al.</a> describe stencil micropatterning-a method that can spatially control stem cell microenvironments and organize their differentiation fates. This elegant method provides a valuable experimental model for tissue organization and patterning in embryonic development.
In <a href="http://www.jove.com/enviro">JoVE Environment</a>, soil plasticity is a concept inspired by the craft of pottery. It refers to the clay-like behavior of certain soils at particular water content levels. The plastic limit of soil is an important parameter in geotechnology, but traditional assessment methods are highly subjective. Thus, <a href="http://www.jove.com/video/54118/">Moreno-Maroto and Alonso-Azc&aacute;rate</a> present an alternative method based on objective measures. This method allows the plastic limit of soil to be obtained with clear and objective criteria.
You've just had a sneak peek of the <a href="http://www.jove.com/archive/112">June 2016 issue</a> of <a href="http://www.jove.com/">JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about June 2016 - This Month in JoVE: Blueberry Analysis, Impact Testing Football Helmets, Stencil Micropatterning of Stem Cells, and a Test for Soil Plasticity at 

June 2016 - This Month in JoVE: Blueberry Analysis, Impact Testing Football Helmets, Stencil Micropatterning of Stem Cells, and a Test for Soil Plasticity

Here's a look at what's coming up in the June 2016 issue of JoVE: The Journal of Visualized Experiments.
In JoVE Chemistry, just in time for blueberry ...

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1.8K Views.  Massachusetts Eye and Ear. June 2016 - This Month in JoVE: Blueberry Analysis, Impact Testing Football Helmets, Stencil Micropatterning of Stem Cells, and a Test for Soil Plasticity

Video: June 2016 - This Month in JoVE: Blueberry Analysis, Impact Testing Football Helmets, Stencil Micropatterning of Stem Cells, and a Test for Soil Plasticity

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...