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Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

7.2K Views

08:12 min

January 3rd, 2019

DOI :

10.3791/58697-v

January 3rd, 2019


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Single step Purification

Rozdziały w tym wideo

0:04

Title

0:51

Substrate Preparation

1:28

Complex Assembly

2:14

Immobilization of the RNA/protein Complexes on Streptavidin Beads

4:00

UV-Elution

5:08

Sample Analysis by Silver Staining Followed by Mass Spectrometry

5:59

Results: Analysis of UV-eluted Complexes

7:36

Conclusion

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