Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
7.2K Views
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08:12 min
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January 3rd, 2019
DOI :
January 3rd, 2019
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Chapters in this video
0:04
Title
0:51
Substrate Preparation
1:28
Complex Assembly
2:14
Immobilization of the RNA/protein Complexes on Streptavidin Beads
4:00
UV-Elution
5:08
Sample Analysis by Silver Staining Followed by Mass Spectrometry
5:59
Results: Analysis of UV-eluted Complexes
7:36
Conclusion
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