An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
10.9K Views
•
09:45 min
•
August 18th, 2018
DOI :
August 18th, 2018
•Rozdziały w tym wideo
0:04
Title
0:32
Antisense Oligonucleotide (ASO) Design
2:22
Protein Extract Preparation
4:08
Step 1: Poly(A) RNA Isolation
5:45
Step 2: Specific mRNA Capture with 3 -Biotinylated 2 -Methoxy Modified Antisense RNA Oligonucleotides
7:41
Results: Capture of Specific mRNA-protein Complexes from Extracts Derived from UV Crosslinked Cells with TRIP
9:00
Conclusion
Powiązane Filmy
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
8.9K Views
Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
11.2K Views
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
9.6K Views
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
14.8K Views
Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
11.8K Views
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
7.2K Views
Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
9.5K Views
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
11.7K Views
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
4.7K Views
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
3.5K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone