Name: preparing a celadonite electron source and estimating its brightness
Uploaded: 2019-11-05T12:30:00.000+00:00
Duration: 9 min 14 s
Description: Watch this Scientific Journal Video about Preparing a Celadonite Electron Source and Estimating Its Brightness at JoVE.com

The authors would like to thank Marjorie Sweetko for improving the English of this article.

1. Preparation of the source
NOTE: In our microscope, the source-support is composed of a machinable glass ceramic plate from which emerges 1 cm of a stainless-steel tube of 90 &#181;m internal diameter with an electrical connection on the plate.
<ol>
	<li>Preparation of the fiber
	<ol>
		<li>Fix the source support under an optical microscope.</li>
		<li>Insert the 10 &#181;m carbon fiber into the stainless-steel tube. Glue the carbon fiber to the tube with silver lacquer.</li>
		<li>Cut the fiber with a cutting tweezers (under a binocular microscope) so that between 100 &#181;m and 3 mm are left outside the stainless-steel tube. 
		NOTE: The carbon fiber is brittle; leaving more than 1 cm outside the tube will increase the chance of the structure breaking during manipulation.</li>
	</ol>
	</li>
	<li>Celadonite-containing water preparation
	<ol>
		<li>Grind the celadonite with a mortar and pestle.</li>
		<li>Weigh 0.2 mg of celadonite powder and dilute in 10 mL of deionized water.</li>
		<li>Use an ultrasound tip directly in the 10 mL of celadonite-containing water to break the aggregates. Typically, use an ultrasonic frequency of 30 kHz for a power of 50 W over 30 s.</li>
	</ol>
	</li>
	<li>Preparation of the deposition environment
	<ol>
		<li>Connect a capillary holder to a pressure controller.</li>
		<li>Maintain the capillary holder under an optical microscope with a multidirectional micro-manipulator.</li>
		<li>Place the support with the carbon fiber facing the capillary holder under the optical microscope.</li>
	</ol>
	</li>
	<li>Celadonite deposition
	<ol>
		<li>Pull a micropipette with an internal-end diameter of 2-10 &#181;m to allow the dispersed celadonite to flow without obstruction.
		<ol>
			<li>Fix a glass capillary in the puller jaw. Ensure the right puller parameters according to patch pipette size (Table 1). Fill the micropipette with the celadonite-containing water.</li>
		</ol>
		</li>
		<li>Mount the micropipette on the capillary holder under the microscope. Align the micropipette and the carbon fiber under the optical microscope.</li>
		<li>Approach the micropipette, to a distance of 2-10 &#181;m from the apex of the carbon fiber.</li>
		<li>Apply progressive pressure on the wide entry to the micropipette. Typically, apply 100 mbar so that a drop forms at the tip but does not fall. This drop wets the apex of the carbon fiber.</li>
		<li>Retract the micropipette.</li>
	</ol>
	</li>
</ol>
2. Kicking-off the source
NOTE: In our microscope, the source-support is fixed on a manual rotating flange also carrying the piezo-electric actuator that moves (100 nm resolution, 25 mm range), with an electrical command, the object relative to the source (See Figure 2). This object plays the role of an electrical anode for electron emission; it is generally electrically grounded and placed in front of the source. In our experiment, voltages are hand controlled with different power supplies.
<ol>
	<li>Install the source holder under vacuum.</li>
	<li>Connect the carbon fiber and the object to two high-voltage electrical feedthroughs.</li>
	<li>Check electrical continuity of contacts everywhere: anode-object, lens and screen; turn on the vacuum pumping.</li>
	<li>Connect a nano-ammeter of a caliber in the &#181;A range between the object and the electrical ground.</li>
	<li>Increase the negative bias voltage applied to the source slowly, at approximately 1 V/s. If the anode is 1 mm away from the source, the kick-off takes place at about 2 kV. Intensity suddenly increases.</li>
	<li>Decrease the voltage to stabilize the intensity at some hundred nA. At the beginning, intensity can fluctuate over several orders of magnitude.</li>
	<li>Leave the system fluctuating for several hours, until fluctuations decrease. Cut off the voltage when fluctuations are lower than 10%.</li>
</ol>
3. Source characterization
NOTE: We present a way to probe the source characteristics. To estimate the source brightness, two projection microscopes are used. In these setups, the shadow of an object is observed on a fluorescent screen placed farther away (Figure 2). The source (cathode) and the object (anode) are mounted on a micro-manipulation flange and can rotate together in the projection plane. A simple short projection setup with a fluorescent screen allows for low magnification projection. The second setup involves an electrostatic lens and a dual microchannel-plate/fluorescent screen assembly for the strongest magnifications12. Information available on each projection image is used to under-estimate the brightness: the smallest detail in the record13. This smallest visible detail depends on the apparent source-size, that includes the source-size geometrical blur, the vibrations between the object and the source, and the detector resolution.
<ol>
	<li>Measurement of the cone angle
	<ol>
		<li>Turn the source towards the simple projection setup, with the rotating flange, to observe the electron beam.</li>
		<li>Decrease the source-to-screen distance, with the manual micro-manipulator, to obtain the entire spot on the screen; then, measure the source-to-screen distance, D.</li>
		<li>Take pictures of the screen by changing the angle between the electron beam and the normal to the screen, with the rotating flange.</li>
		<li>Plot the gray-level intensity profile along one axis and determine the emission radius, R at a given source-to-screen distance, D (Figure 3).</li>
		<li>Calculate the cone angle: <img src="/files/ftp_upload/59513/59513eq1.jpg" /> with R, the emission radius at a given source-to-screen distance, D.</li>
	</ol>
	</li>
	<li>Measurement of the Fowler-Nordheim plot
	<ol>
		<li>Measure the emission intensity versus the voltage applied to the source: I(V) with I the intensity measured at the anode and V the voltage applied at the carbon fiber.</li>
		<li>Plot <img src="/files/ftp_upload/59513/59513eq2.jpg" />. The curve shows a decreasing straight line with saturation for highest voltage. An example is given in Figure 4. The longest straight line is the signature of the field emission process.</li>
	</ol>
	</li>
	<li>Measurement of the source size
	<ol>
		<li>Turn the source towards the electrostatic lens, with the rotating flange.</li>
		<li>Produce a projection image containing a huge Fresnel diffraction pattern along an edge of an object: magnification of about 20,000x is required. In our microscope, this is possible with a source-to-object distance of some 100 &#181;m, fixed with the piezo-actuators, and an Einzel electrostatic lens.</li>
		<li>Measure the sharpest visible detail on the image on the screen (Figure 5). 
		NOTE: The sharpest fringe-to-fringe distance, &#948;, is used.</li>
		<li>Calculate the source size: <img src="/files/ftp_upload/59513/59513eq3.jpg" />.</li>
	</ol>
	</li>
</ol>

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	<li>Forbes, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low-macroscopic-field+electron+emission+from+carbon+films+and+other+electrically+nanostructured+heterogeneous+materials:+hypotheses+about+emission+mechanism.">Low-macroscopic-field electron emission from carbon films and other electrically nanostructured heterogeneous materials: hypotheses about emission mechanism.</a> Solid-State Electronics. 45, 779-808 (2001).</li><li>Wang, C., Garcia, A., Ingram, D. C., Lake, M., Kordesch, M. 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W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+surface+electron-emission+mechanism+in+diamond+cathodes.">A new surface electron-emission mechanism in diamond cathodes.</a> Nature. 393, 431-435 (1998).</li><li>Horch, S., Morin, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Field+emission+from+atomic+size+sources.">Field emission from atomic size sources.</a> Journal of Applied Physics. 74 (6), 3652-3657 (1993).</li><li>Muller, H. U., Volkel, B., Hofmann, M., Woll, C., Grunze, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Emission+properties+of+electron+point+sources.">Emission properties of electron point sources.</a> Ultramicroscopy. 50 (1), 57-64 (1993).</li><li>Qian, W., Scheinfein, M. R., Spence, J. C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brightness+measurements+of+nanometer-sized+field-emission-electron+sources.">Brightness measurements of nanometer-sized field-emission-electron sources.</a> Journal of Applied Physics. 73 (11), 7041-7045 (1993).</li><li>Rech, J. e. m., Grauby, O., Morin, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low-voltage+electron+emission+from+mineral+films.">Low-voltage electron emission from mineral films.</a> Journal of Vacuum Science &amp; Technology B. 20 (1), 5-9 (2002).</li><li>Daineche, R., Degiovanni, A., Grauby, O., Morin, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Source+of+low-energy+coherent+electron+beams.">Source of low-energy coherent electron beams.</a> Applied Physics Letters. 88, 023101 (2006).</li><li>Salan&#231;on, E., Daineche, R., Grauby, O., Morin, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single+mineral+particle+makes+an+electron+point+source.">Single mineral particle makes an electron point source.</a> Journal of Vacuum Science &amp; Technology B. 33, 030601 (2015).</li><li>Prigent, M., Morin, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Charge+effect+in+point+projection+images+of+Ni+nanowires+and+I+collagen+fibres.">Charge effect in point projection images of Ni nanowires and I collagen fibres.</a> Journal of Physics D: Applied Physics. 34 (8), 1167-1177 (2001).</li><li>Salan&#231;on, E., Degiovanni, A., Lapena, L., Lagaize, M., Morin, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+low-energy+electron+point-source+projection+microscope+not+using+a+sharp+metal+tip+performs+well+in+long-range+imaging.">A low-energy electron point-source projection microscope not using a sharp metal tip performs well in long-range imaging.</a> Ultramicroscopy. 200, 125-131 (2019).</li><li>Salan&#231;on, E., Degiovanni, A., Lapena, L., Morin, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+spatial+resolution+detection+of+low-energy+electrons+using+an+event-counting+method,+application+to+point+projection+microscopy.">High spatial resolution detection of low-energy electrons using an event-counting method, application to point projection microscopy.</a> Review of Scientific Instruments. 89, 043301 (2018).</li><li>Swanson, L. 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The authors have no competing financial interests.

This protocol is not critical because the geometry of the source at a microscopic scale changes from one source to another one. The difficulty is that since a carbon fiber is brittle, its cutting can lead to an inappropriate length. An adequate length is about 500 &#181;m; the microscopic shape of the cut is not crucial. The critical step is to have a very small number of crystals (ideally one) deposited on the apex of a conductive wire. Adapting the crystal concentration with the deposited volume is the most important p...

Metal/insulator structures used for electron emission have been studied for almost 20 years due to their low macroscopic field1. The electric field involved is only of the order of some V/&#181;m2,3,4, in contrast to the V/nm required for classic field emission with sharp metal tips5,6,7. This probably explains the starting plasma discharges that are so useful in electron source technologies. Some years ago, we sought to explore this low field emission by depositing films of natural insulators on electron transmission carbon layers8. Celadonite, an insulator mineral found in the basalt of the Parana Traps in the mines of Ametista di Sul in Brazil, was chosen.
When celadonite is ground, the crystal shape is a rectangular slab with micrometric dimensions and a thickness of less than 100 nm (typically: 1,000 nm x 500 nm x 50 nm). It is perfectly flat and recognizable in scanning electron microscopy (Figure 1). The film is formed by deposition of a celadonite-containing water droplet on the carbon layer. As applied voltage increases, it emits electrons following a Fowler-Nordheim regime with intensity saturation for the highest voltages. A study using a diaphragm in a projection system showed that one emitter is a point-like source9. However, using this large film with a diaphragm to select the source did not exploit the potential of the point-source. For example, the point-sources commonly used in low-energy electron point-source projection microscopy allow a source-to-object distance of about 100 nm. However, such a source-to-object distance would be out of the question with a film. Finding a way to isolate one crystal so as to be able to move something towards this electron source was a challenge. Our solution was first, to use a 10 &#181;m carbon fiber: depositing the droplet at the apex of the fiber necessarily limits the number of celadonite crystals. Second, we decided to limit droplet size: a micropipette with a tip end of about 5 &#181;m is filled with celadonite-containing water and pressure is applied at the entrance of the micropipette to create a small drop to wet the apex of the fiber. The protocol details the full source preparation process.
The resulting source is a coaxial point-source allowing good alignment between the source, the object and the electron optical system10. Because its 10 &#181;m diameter is still wider than ultra-sharp tips, the source-to-object distance is limited to some tens of micrometers. However, we recently showed that the celadonite source emitter combined with an Einzel lens performs comparably to a classic point-source projection microscope. The long-range imaging thus made accessible even limits the charge effect11 on the object and the image distortions involved12,13. The celadonite source also presents major advantages compared to sharp metal tips. It is robust: the point-source is under the crystal and thus protected against sputtering. The source can operate under relatively high pressure: it was tested at 10-2 mbar during some minutes. Yet its lifetime and its stability remain dependent on the right vacuum conditions. We usually employ the celadonite source at 10-8 mbar and obtain a lifetime of months.
This article is intended to help all those wishing to use the celadonite source to produce a coherent electron beam.

<table><tbody><tr><td>Carbon fiber filament</td><td>Goodfellow</td><td>C 005711&amp;nbsp;&amp;nbsp;</td><td></td></tr><tr><td>Carbon fiber filament</td><td>Mitsubishi Chemical</td><td>DIALEAD</td><td></td></tr><tr><td>Carbon fiber filament</td><td>Solvay</td><td>THORNEL P25</td><td></td></tr><tr><td>Carbon fiber filament</td><td>Zoltek</td><td>PX35 Continuous Tow</td><td></td></tr><tr><td>Celadonite</td><td></td><td></td><td>Verona Green earth / pigment</td></tr><tr><td>Dual-stage microchannel plate and fluorescent screen assembly</td><td>Hamamatsu</td><td>F2225-21S</td><td></td></tr><tr><td>Flow controller</td><td>Elveflow</td><td>OB1&amp;nbsp;</td><td></td></tr><tr><td>Machinable glass ceramic</td><td>Macor</td><td></td><td></td></tr><tr><td>Micropipette Puller</td><td>Sutter Instruments</td><td>P2000&amp;nbsp;</td><td></td></tr><tr><td>Piezo-electric actuators</td><td>Mechonics</td><td>MS30&amp;nbsp;</td><td></td></tr><tr><td>Quartz capillary</td><td>Sutter Instrument&amp;nbsp;</td><td>B100-75-15&amp;nbsp;</td><td></td></tr><tr><td>Silver Lacquer</td><td>DODUCO GmbH</td><td>AUROMAL 38&amp;nbsp;&amp;nbsp;</td><td></td></tr><tr><td>Ultrasonic processor</td><td>Hielscher / sonotrode MS3</td><td>UP50H&amp;nbsp;</td><td></td></tr></tbody></table>

preparing a celadonite electron source and estimating its brightness

The electron celadonite source described here performs well in a low-energy electron point-source projection microscope in long-range imaging. It presents major advantages compared to sharp metal tips. Its robustness affords a lifetime of months and it can be used under relatively high pressure. The celadonite crystal is deposited at the apex of a carbon fiber, maintained itself in a coaxial structure ensuring a spherical beam shape and easy mechanical positioning to align the source, the object and the electron-optical system axis. There is a single crystal deposition via generation of celadonite-containing water droplets with a micropipette. Scanning electron microscopy observation can be performed to verify the deposition. However, this adds steps and therefore increases the risk of damaging the source. Thus, after preparation, the source is usually inserted directly under vacuum in the projection microscope. A first high voltage supply provides the kick-off needed to start the electron emission. The field emission process involved is then measured: it has already been observed for dozens of electron sources prepared in this way. The brightness is under-estimated through an over-estimation of source size, intensity at one energy and cone angle measured in a projection system.

Several scanning electron micrographies of carbon fibers prepared as detailed in protocol were obtained in an SEM at 15 kV. Sources exhibit one, sometimes two, crystals at their apex (Figure 1). However, the use of the SEM involves another support for the carbon fiber, which is hard to mount and demount without breaking. It is safer to attempt direct electron emission. Tested in a projection microscope (Figure 2), every source pr...

Watch this Scientific Journal Video about Preparing a Celadonite Electron Source and Estimating Its Brightness at JoVE.com

Preparing a Celadonite Electron Source and Estimating Its Brightness

The article presents a protocol to prepare a celadonite source and estimate its brightness for use in a long-range imaging low-energy electron point-source projection microscope.

The electron celadonite source described here performs well in a low-energy electron point-source projection microscope in long-range imaging. It presents ...

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4.5K Views.  Aix-Marseille University, CNRS. The article presents a protocol to prepare a celadonite source and estimate its brightness for use in a long-range imaging low-energy electron point-source projection microscope.

Video: Preparing a Celadonite Electron Source and Estimating Its Brightness

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The achromatic doublet on glass (ADG) Fresnel lens makes use of two materials with differing dispersion to reduce chromatic aberration and increase attainable concentration. In this paper, a protocol for the complete characterization of the ADG Fresnel lens is presented.

We present a method to characterize achromatic Fresnel lenses for photovoltaic applications. The achromatic doublet on glass (ADG) Fresnel lens is ...

Preparation and Characterization of C60/Graphene Hybrid Nanostructures

Physical thermal deposition in a high vacuum environment is a clean and controllable method for fabricating novel molecular nanostructures on graphene. We present methods for depositing and passively manipulating C60 molecules on rippled graphene that advance the pursuit of realizing applications involving 1D C60/graphene hybrid structures. The techniques applied in this exposition are geared towards high vacuum systems with preparation areas capable of supporting molecular deposition as well as thermal annealing of the samples. We focus on C60 deposition at low pressure using a homemade Knudsen cell connected to a scanning tunneling microscopy (STM) system. The number of molecules deposited is regulated by controlling the temperature of the Knudsen cell and the deposition time. One-dimensional (1D) C60 chain structures with widths of two to three molecules can be prepared via tuning of the experimental conditions. The surface mobility of the C60 molecules increases with annealing temperature allowing them to move within the periodic potential of the rippled graphene. Using this mechanism, it is possible to control the transition of 1D C60 chain structures to a hexagonal close packed quasi-1D stripe structure.

Preparation and Characterization of C60/Graphene Hybrid Nanostructures

Here we present a protocol for the fabrication of C60/graphene hybrid nanostructures by physical thermal evaporation. Particularly, the proper manipulation of deposition and annealing conditions allow the control over the creation of 1D and quasi 1D C60 structures on rippled graphene.

Physical thermal deposition in a high vacuum environment is a clean and controllable method for fabricating novel molecular nanostructures on graphene. We ...

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

This video presents the use of transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. These cell lines, after treatment with ascorbic acid (AA) and &#946;-glycerophosphate (&#946;-GP), undergo complete osteogenic transdifferentiation from proliferation to mineralization and produce matrix vesicles (MVs) that trigger apatite nucleation in the extracellular matrix (ECM).
Based on Alizarin Red-S (AR-S) staining and analysis of the composition of minerals in cell lysates using ultraviolet (UV) light or in vesicles using TEM imaging followed by EDX quantitation and ion mapping, we can infer that osteosarcoma Saos-2 and osteoblastic hFOB 1.19 cells reveal distinct mineralization profiles. Saos-2 cells mineralize more efficiently than hFOB 1.19 cells and produce larger mineral deposits that are not visible under UV light but are similar to hydroxyapatite (HA) in that they have more Ca and F substitutions.
The results obtained using these techniques allow us to conclude that the process of mineralization differs depending on the cell type. We propose that, at the cellular level, the origin and properties of vesicles predetermine the type of minerals.

We present a protocol to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. Their mineralization profiles were analyzed by Alizarin Red-S (AR-S) staining, ultraviolet (UV) light visualization, transmission electron microscopy (TEM) imaging and energy dispersive X-ray microanalysis (EDX).

This video presents the use of transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) to compare the state of minerals in ...

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

There is a need to understand materials exposed to overlapping extreme environments such as high temperature, radiation, or mechanical stress. When these stressors are combined there may be synergistic effects that enable unique microstructural evolution mechanisms to activate. Understanding of these mechanisms is necessary for the input and refinement of predictive models and critical for engineering of next generation materials. The basic physics and underlying mechanisms require advanced tools to be investigated. The in situ ion irradiation transmission electron microscope (I&#179;TEM) is designed to explore these principles.
To quantitatively probe the complex dynamic interactions in materials, careful preparation of samples and consideration of experimental design is required. Particular handling or preparation of samples can easily introduce damage or features that obfuscate the measurements. There is no one correct way to prepare a sample; however, many mistakes can be made. The most common errors and things to consider are highlighted within. The I&#179;TEM has many adjustable variables and a large potential experimental space, therefore it is best to design experiments with a specific scientific question or questions in mind.
Experiments have been performed on large number of sample geometries, material classes, and with many irradiation conditions. The following are a subset of examples that demonstrate unique in situ capabilities utilizing the I3TEM. Au nanoparticles prepared by drop casting have been used to investigate the effects of single ion strikes. Au thin films have been used in studies on the effects of multibeam irradiation on microstructure evolution. Zr films have been exposed to irradiation and mechanical tension to examine creep. Ag nanopillars were subjected to simultaneous high temperature, mechanical compression, and ion irradiation to study irradiation induced creep as well. These results impact fields including: structural materials, nuclear energy, energy storage, catalysis, and microelectronics in space environments.

Sample preparation techniques are outlined with specific considerations for in situ ion irradiation TEM experiments. Ion species, energy, and fluence are discussed with methods for how to select and compute them. Finally, procedures for conducting an experiment are described and accompanied by the representative results.

There is a need to understand materials exposed to overlapping extreme environments such as high temperature, radiation, or mechanical stress. When these ...

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Intraoperative imaging techniques have the potential to make surgical interventions safer and more effective; for these reasons, such techniques are quickly moving into the operating room. Here, we present a new approach that utilizes a technique not yet explored for intraoperative imaging: chemiluminescent imaging. This method employs a ruthenium-based chemiluminescent reporter along with a custom-built nebulizing system to produce ex vivo or in vivo images with high signal-to-noise ratios. The ruthenium-based reporter produces light following exposure to an aqueous oxidizing solution and re-reduction within the surrounding tissue. This method has allowed us to detect reporter concentrations as low as 6.9 pmol/cm2. In this work, we present a visual guide to our proof-of-concept in vivo studies involving subdermal and intravenous injections in mice. The results suggest that this technology is a promising candidate for further preclinical research and might ultimately become a useful tool in the operating room.

This protocol describes a new intraoperative imaging technique that uses a ruthenium complex as a source of chemiluminescent light emission, thereby producing high signal-to-noise ratios during in vivo imaging. Intraoperative imaging is an expanding field that could revolutionize the way that surgical procedures are performed.

Intraoperative imaging techniques have the potential to make surgical interventions safer and more effective; for these reasons, such techniques are ...