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Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

4.3K Views

09:24 min

May 9th, 2020

DOI :

10.3791/60799-v

May 9th, 2020


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Cancer Stem Cells

Rozdziały w tym wideo

0:04

Introduction

1:02

Generation of Inducible Knockdown GCSCs Lines

3:31

Generation of Stable Transfected Cell Lines

6:12

Sphere Formation Assay

6:54

Results: Inducible Knockdown of Clusterin Inhibits GCSC Self-renewal Capacity

8:22

Conclusion

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