Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

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9.9K Views

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07:01 min

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March 1st, 2020

DOI :

10.3791/60022-v

March 1st, 2020

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Mafalda Laranjo*¹^,²^,³^,⁴, Maria João Carvalho*¹^,²^,³^,⁴^,⁵^,⁶, Beatriz Serambeque¹^,²^,³, André Alves²^,⁷, Carlos Miguel Marto¹^,²^,³^,⁴^,⁸, Isabel Silva⁹, Artur Paiva³^,⁹^,¹⁰, Maria Filomena Botelho¹^,²^,³^,⁴^,⁸

¹Institute of Biophysics, Faculty of Medicine, University of Coimbra, ²Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, ³CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, ⁴Clinical Academic Center of Coimbra (CACC), ⁵Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, ⁶Gynecology A Service, Coimbra Hospital and Universitary Center, ⁷Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, ⁸Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, ⁹Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, ¹⁰Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences

Podsumowanie

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Cancer Stem Cells

Tumor Isolation

Phenotypic Characterization

Epidermal Growth Factor

Basic Fibroblast Growth Factor

Three dimensional Colonies

Adherent Populations

Sphere forming Capacity

Rozdziały w tym wideo

0:05

Introduction

0:35

Sphere Formation and Derived Adherent Population Generation

2:28

Sphere-forming Capacity Determination

3:02

Self-renewal Determination

3:59

Sphere Projection Area Determination

4:51

Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses

6:29

Conclusion

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