Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

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1.3K Views

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08:24 min

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July 27th, 2021

DOI :

10.3791/61689-v

July 27th, 2021

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Jing Zhang*¹, Jing Huang*², Ishani Majumdar¹, Ryan C. James³, Julia Gichimu¹, Manikandan Paramasivam⁴, Durga Pokharel⁵, Himabindu Gali⁶, Marina A. Bellani¹, Michael M Seidman¹

¹Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, ²Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, ³Department of Molecular Biology and Genetics, Cornell University, ⁴Department of Cellular and Molecular Medicine, University of Copenhagen, ⁵Horizon Discovery, ⁶Boston University School of Medicine

Podsumowanie

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Replisome Visualization

Antigen tagged DNA

Blocking Lesion

Immunodetection

Cell Cycle Interactions

Ishani Majumdar

Seidman Laboratory

Cell Culture Protocol

UVA Exposure

Cytoskeleton Extraction Buffer

Formaldehyde Fixation

Triton X 100 Treatment

Rozdziały w tym wideo

0:04

Introduction

0:51

Cell Preparation

3:58

Proximity Ligation Assay (PLA)

6:20

Results: Representative Replisome Encounter Visualization

7:52

Conclusion

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