Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
1.3K Views
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08:24 min
•
July 27th, 2021
DOI :
July 27th, 2021
•Jing Zhang*1, Jing Huang*2, Ishani Majumdar1, Ryan C. James3, Julia Gichimu1, Manikandan Paramasivam4, Durga Pokharel5, Himabindu Gali6, Marina A. Bellani1, Michael M Seidman1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, 3Department of Molecular Biology and Genetics, Cornell University, 4Department of Cellular and Molecular Medicine, University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
Chapters in this video
0:04
Introduction
0:51
Cell Preparation
3:58
Proximity Ligation Assay (PLA)
6:20
Results: Representative Replisome Encounter Visualization
7:52
Conclusion
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