Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

1.3K Views

•

08:24 min

•

July 27th, 2021

DOI :

10.3791/61689-v

July 27th, 2021

•

Jing Zhang*¹, Jing Huang*², Ishani Majumdar¹, Ryan C. James³, Julia Gichimu¹, Manikandan Paramasivam⁴, Durga Pokharel⁵, Himabindu Gali⁶, Marina A. Bellani¹, Michael M Seidman¹

¹Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, ²Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, ³Department of Molecular Biology and Genetics, Cornell University, ⁴Department of Cellular and Molecular Medicine, University of Copenhagen, ⁵Horizon Discovery, ⁶Boston University School of Medicine

Summary

Explore More Videos

Replisome Visualization

Antigen tagged DNA

Blocking Lesion

Immunodetection

Cell Cycle Interactions

Ishani Majumdar

Seidman Laboratory

Cell Culture Protocol

UVA Exposure

Cytoskeleton Extraction Buffer

Formaldehyde Fixation

Triton X 100 Treatment

Chapters in this video

0:04

Introduction

0:51

Cell Preparation

3:58

Proximity Ligation Assay (PLA)

6:20

Results: Representative Replisome Encounter Visualization

7:52

Conclusion

Related Videos

08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

13.1K Views

07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

33.0K Views

07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

14.1K Views

12:48

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

14.7K Views

18:27

One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bis(sulfosuccinimidyl) Suberate (BS3)

18.7K Views

13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

10.0K Views

08:14

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

11.0K Views

08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

10.7K Views

09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

8.7K Views

13:26

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

9.7K Views