A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

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3.2K Views

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07:56 min

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August 11th, 2021

DOI :

10.3791/62127-v

August 11th, 2021

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Jose A. Pino*¹, Nora Awadallah*², Alessandra M. Norris³, Gonzalo E. Torres²

¹Department of Medicine, School of Medicine, Universidad de Atacama, ²Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, ³Department of Pharmacology and Therapeutics, University of Florida College of Medicine

Podsumowanie

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Plate based Assay

Monoamine Release

Brain Slices

Pharmacological Conditions

Endogenous Monoamine Release

Dorsal Striatum

Hippocampus

Efflux Chamber

Oxygenated Dissection Buffer

Tissue Samples

Incubation Period

Pharmacological Agent

Rat Brain Atlas

Microcentrifuge Tubes

Rozdziały w tym wideo

0:04

Introduction

0:44

Acute Rat Brain Slice Preparation

2:01

Ex Vivo Endogenous Monoamine Release Induction

4:42

HPLC Analysis

5:29

Results: Representative Endogenous Monoamine Release in Acute Brain Slices

7:21

Conclusion

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