Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

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4.7K Views

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06:26 min

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May 16th, 2021

DOI :

10.3791/62469-v

May 16th, 2021

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Misbha Ud Din Ahmad¹, Alexander Fish¹, Jeroen Molenaar¹, Sridhar Sreeramulu², Christian Richter², Nadide Altincekic², Harald Schwalbe², Hans Wienk¹, Anastassis Perrakis¹

¹Oncode Institute and Division of Biochemistry, the Netherlands Cancer Institute, ²Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University

Podsumowanie

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Nano Differential Scanning Fluorimetry

Fragment based Lead Discovery

Protein Targets

Ligand Screening

Protein Stock Solution

MRC 2 well Plate

Graphical User Interface

Adhesive Sealing Film

Rozdziały w tym wideo

0:04

Introduction

0:35

Plate Preparation

1:06

Fragment Nano-Dispensing by the Mosquito Robot

2:01

Fragment Dispensing

3:17

Preparing the Prometheus

4:25

Fluorescence Scan and Thermal Denaturation

5:04

Results: Fragment Screening

5:49

Conclusion

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