High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening
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10:01 min
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March 31st, 2022
DOI :
March 31st, 2022
•Yu-Fen Chang1, Wan-Chi Su1, Chih-Chuan Su1, Min-Wen Chung1, Jin Chang2, You-Yi Li3, Yi-Ju Kao3, Wen-Pin Chen3, Matthew J. Daniels4,5,6
1LumiSTAR Biotechnology, Inc., 2NEXEL Co., Ltd., 3Institute of Pharmacology, College of Medicine, National Taiwan University, 4Manchester Heart Centre, Manchester Royal Infirmary, Manchester University NHS Foundation Trust, 5Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre, University of Manchester, 6Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester
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Rozdziały w tym wideo
0:04
Introduction
0:57
iPSC Derived Cardiomyocytes Preparation
2:28
Expression of Genetically Encoded Calcium Indicators
3:24
Image Acquisition and Functional Assays for Toxicity Test and Phenotypic Screening
5:22
Small Molecule Preparation
5:58
Imaging Processing and Analysis
6:31
Results: Imaging and Processing of Cells
9:28
Conclusion
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