High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

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3.0K Views

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06:39 min

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April 21st, 2022

DOI :

10.3791/63202-v

April 21st, 2022

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Bruce Nguyen Tran¹, Eunkeu Oh², Kimihiro Susumu²^,³, Mason Wolak², Kirill Gorshkov¹

¹National Center for Advancing Translational Sciences, ²Optical Sciences Division, Code 5600, Naval Research Laboratory, ³Jacobs Corporation

Podsumowanie

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High throughput Imaging

Cellular Applications

0 1 BSA Preparation

QD Spike Solution

Multi channel Pipette

Rozdziały w tym wideo

0:04

Introduction

0:30

Treatment of Cells with QD-Spike

1:39

Fixation and Nuclei Staining

2:36

Acquisition Set-Up and Imaging

4:39

Results: Imaging and Visualization of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers

6:11

Conclusion

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