Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

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1.7K Views

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09:41 min

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December 28th, 2021

DOI :

10.3791/63271-v

December 28th, 2021

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Meng-Fei Xu¹^,², Yu-Hao Yang¹^,², Ya-Lan Wei³^,⁴, Jing-Lian Zhang¹^,², Xuan Lin¹^,², Xiao-Yan Lin¹^,², Hui Chen¹^,², Zhen-Yu She¹^,²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, ³Fujian Obstetrics and Gynecology Hospital, ⁴Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

Podsumowanie

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Paraffin Solidification

Rozdziały w tym wideo

0:05

Introduction

1:13

Construction of GSK923295-Mediated Centromere Protein E (CENP-E) Inhibition Mouse Models

2:18

Hematoxylin-eosin (HE) Staining and Histopathology

3:57

Immunofluorescence and Confocal Microscopy

5:31

Flow Cytometry

7:04

Results: Analysis of Seminiferous Tubules and the Disruption of Spermatogenesis Post CENP-E Inhibition by GSK923295

8:59

Conclusion

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